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KMID : 0620920020340010012
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Experimental & Molecular Medicine
2002 Volume.34 No. 1 p.12 ~ p.17
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The stimulatory effect of IL-1β on the insulin secretion of rat pancreatic islet is not related with iNOS pathway
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Kwang Won Kim/In Kyung Jeong
Seung Hoon Oh/Jae Hoon Chung/Yong Ki Min/Myung Shik Lee/Moon Kyu Lee/Kwang Won Kim
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Abstract
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Interleukin 1 (IL-1) is a pleiotropic cytokine with the potential to destroy pancreatic β-cells, and thought to be involved in the pathogenesis of type I diabetes mellitus. Expression of inducible nitric oxide synthase (iNOS) and subsequent NO formation induced by IL-1β may impair an islet function in rodents. Inhibition of iNOS may protect against cytokine-induced β-cell suppression, although cytokines might also induce NO-independent impairment. To examine the role of NO in the IL-1β treated cells, rat islets were treated with various concentrations (0, 0.5, 5, 50, 500 pmol/L) of IL-1β with or without NG-monomethyl-L-arginine (NMMA; a competitive inhibitor of nitiric oxide synthase) for 2 or 6 h. Insulin secretion was stimulated in islets treated with 5, 50, and 500 pmol/ L of IL-1β for 2 h and 0.5 pmol/L for 6 h, respectively. The stimulatory effect of IL-1β on the insulin secretion of rat islets was not prevented by NMMA. Nitrate concentration was increased in a time- and concentration-dependent manner. Nitrate production was inhibited by NMMA. iNOS mRNA expression was increased at concentrations more than 5 pmol/L of IL-1β in a dose dependent manner. iNOS mRNA was detectable after 2 h and peaked at 6 h but decreased after 24 h. These results suggested that the stimulatory effect of IL-1β on the insulin secretion of rat islets is independent of iNOS-related NO production of IL-1β and the enzyme activity of nitric oxide synthase.
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KEYWORD
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Interleukin-1β, islet, insulin, inducible nitric oxide synthase(iNOS), nitric oxide,
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